Updated dailyPubMed-sourced · Research, not medical advice
Reference10unitsU-100 equivalent for the sample amount
0.1 mLCalculated volume
5 mg/mLConcentration
50 mcgPer syringe unit
20Samples per vial

Laboratory supplies commonly used for dilution

Sterile Diluent

Laboratory diluent used to reconstitute lyophilized research compounds. Look for sealed, clearly labeled and in-date supplies.

Labels & Cold Storage

Sample labels, vial racks and temperature-aware storage supplies make research handling easier to track and audit.

How peptide reconstitution math works

Reconstitution is simply dissolving a freeze-dried (lyophilized) peptide in bacteriostatic water so it can be measured and drawn. The only math involved is concentration and volume:

  1. Concentration = peptide in the vial (mg) ÷ water added (mL). A 10 mg vial in 2 mL of water is 5 mg/mL.
  2. Calculated volume = the sample amount ÷ the concentration. For a 500 mcg (0.5 mg) sample at 5 mg/mL, that's 0.1 mL.
  3. U-100 reference units = volume (mL) × 100. So 0.1 mL = 10 reference units.

More diluent makes each sample a larger, easier-to-read volume; less diluent concentrates it into fewer units. The calculator above updates all of this live as you change any input.

⚕️ This calculator is an educational arithmetic tool only. It does not recommend a dose and is not medical advice. Many peptides are not approved for human use and are sold for research purposes only. Always consult a qualified healthcare professional before making any decision about your health.

New to a specific peptide? Read the evidence-based guides or compare peptides in the Finder.